How does Cre-LOX work?
How does Cre-LOX work?
General principle of Cre-loxP system Concerning the mechanism of Cre-loxP system, a single Cre recombinase recognizes two directly repeated loxP site, then the Cre excises the loxP flanked (floxed) DNA, thus creating two types of DNA with circular, excised and inactivated gene Y (Figure 1A).
Why is Cre-lox important?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
What does Cre-LOX mean?
The Cre-lox system is a technology that can be used to induce site-specific recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site.
What does the Cre protein do?
Cre recombinase plays important roles in the life cycle of the P1 bacteriophage. Upon infection of a cell the Cre-loxP system is used to cause circularization of the P1 DNA. In addition to this Cre is also used to resolve dimeric lysogenic P1 DNA that forms during the cell division of the phage.
How are cre lox mice generated?
In general, Cre/lox mice are generated by mating a Cre-driver mouse with a flox mouse.
Is Cre lox reversible?
Cre/loxP-mediated inversion of genomic DNA segments represents a useful tool to alter the mouse genome in a cell type- and/or tissue-specific manner. However, its applicability is hampered by the fact that this reaction is fully reversible.
What does Cre stand for?
carbapenem-resistant enterobacteriaceae
CRE, which stands for carbapenem-resistant enterobacteriaceae, are strains of bacteria that are resistant to carbapenem, a class of antibiotics typically used as a last resort for treating severe infections when other antibiotics have failed.
What Cre means?
carbapenem-resistant Enterobacterales
CRE stands for carbapenem-resistant Enterobacterales. Enterobacterales are an order of germs, specifically bacteria. Many different types of Enterobacterales can develop resistance, including Klebsiella pneumoniae and Escherichia coli (E. coli).
Is Cre a restriction enzyme?
Cre recom- bines the short linear segment into the circular DNA episomes, thereby lin- earizing the episome (Figure 1). Hence, Cre can act like a restriction enzyme that recognizes a 34-bp recognition se- quence.
What is the function of Cre recombinase?
In vivo, the function of Cre recombinase is to circularize the P1 genome during infection and maintain the genome in the monomeric state for cell division. Cre-lox recombination has also been extensively employed in in vivo genetic engineering applications in a variety of organisms.
How does tamoxifen induce Cre?
It utilizes a mutated estrogen receptor (ER) fused to Cre as a transgene (Cre-ER), which only becomes activated and then translocates into the nucleus upon binding of the active tamoxifen (TAM) metabolite 4-hydroxytamoxifen (4-OHT), which mutates the ER.
What is the name of the site where Cre enzyme acts?
the LOX site
What is the name of the site where Cre enzyme acts? Explanation: The recombination site on the DNA where Cre acts are called the LOX site.
How are Cre mice generated?
In this system, a region of interest flanked by two lox sites (floxed) is deleted or inverted by Cre-mediated recombination, leading to gene knockout only in a Cre-expressing cell. In general, Cre/lox mice are generated by mating a Cre-driver mouse with a flox mouse.
What is recombinase protein?
Recombinase A (RecA), a naturally aggregating protein involved in DNA repair, is an important asset to the genetic integrity of the Escherichia coli (E. coli) genome. The survival of all species rely on such DNA repair processes.
What is Cre sequence?
The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.
What kind of infection is CRE?
CRE stands for carbapenem-resistant Enterobacterales. Enterobacterales are an order of germs, specifically bacteria. Many different types of Enterobacterales can develop resistance, including Klebsiella pneumoniae and Escherichia coli (E. coli).
What causes CRE infection?
What causes CRE? High and sometimes excessive use of antibiotics promotes the growth of bacteria like CRE. If you have a bacterial infection, your healthcare provider may treat it with an antibiotic. The medicine will work if you have a nonresistant strain of the bacteria.
How does CRE become resistant?
CRE can also become resistant to carbapenems through a combination of chromosomal mutations and acquired non-carbapenemase resistance mechanisms (e.g., a chromosomal mutation in a porin gene that limits the ability of carbapenems to get into the bacteria combined with acquisition or upregulation of a beta-lactamase).
What is the difference between CPE and CRE?
While CRE poses challenges with treatment in general, CPE is considered to be a more significant concern for both infection prevention and treatment since carbapenemase genes are mostly carried on plasmids that have the ability to transfer between bacterial species.