What is PGM sequencing?

What is PGM sequencing?

The Ion PGM System is a reliable sequencing platform that combines simple sample preparation and data analysis solutions with flexible chip output for ultimate project flexibility.

What is DNA target sequence?

Targeted sequencing is a rapid and cost-effective way to detect known and novel variants in selected sets of genes or genomic regions. Gene sequencing can be accomplished using several different DNA sequencing methods, depending on the scale.

What is nanopore sequencing technology?

Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.

What type of sequencing is Illumina?

massively parallel sequencing
Illumina sequencing instruments and reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.

What are the benefits of nanopore sequencing?

Nanopore sequencing represents a robust technology in the DNA sequencing field, producing incredibly long-read sequence data far cheaper and faster than was previously possible. A major advantage of nanopore sequencing is the ability to produce ultra-long reads, and over 2 Mb read lengths have been achieved.

What is a nanopore used for?

What is PCR used for?

PCR (polymerase chain reaction) tests are a fast, highly accurate way to diagnose certain infectious diseases and genetic changes. The tests work by finding the DNA or RNA of a pathogen (disease-causing organism) or abnormal cells in a sample.

What does 5 to 3 direction mean?

DNA is always synthesized in the 5′-to-3′ direction, meaning that nucleotides are added only to the 3′ end of the growing strand. As shown in Figure 2, the 5′-phosphate group of the new nucleotide binds to the 3′-OH group of the last nucleotide of the growing strand.

Does Illumina use fluorescence?

Although the fluorescent imaging system used in Illumina sequencers is not sensitive enough to detect the signal from a single template molecule, the major innovation of the Illumina method is the amplification of template molecules on a solid surface.

What is P5 and P7 Illumina?

The P5 region is cleaved, resulting in clusters containing only fragments which are attached by the P7 region. This ensures that all copies are sequenced in the same direction. The sequencing primer anneals to the P5 end of the fragment, and begins the sequencing by synthesis process.

Why is it called P5 and P7?

P5 and P7 are names given by Illumina to the oligo sequences that bind to the flow cell. High throughput sequencing is frequently used to discover the location of regulatory interactions on chromatin.

Is Illumina or nanopore more accurate?

Moreover, nanopore sequencing has 92-97% accuracy, while illumina sequencing has 99% accuracy. The following infographic lists the differences between nanopore and illumina sequencing in tabular form.

What are the disadvantages of nanopore sequencing?

Nanopore sequencing does have its disadvantages. It tends to be error prone; error rates in nanopore sequencing can be as high as 15%. If sequencing large amounts of the same sequence, this error rate can be tolerable because multiple copies of the same sequence will allow the user to recognize and eliminate mistakes.