What is base deactivated silica?

What is base deactivated silica?

Base deactivating was first a kind of chemical cleaning procedure (“wash”) of the base silica bofore making the bonding process. This procedure help to reduce the metal content of the silica surface and so reducing the acidity.

What is the meaning of LiChrosorb in chromatography?

LiChrosorb is a reliable irregular shaped Type A silica sorbent with a particle size of 5um, 7um or 10um. A broad range of modifications on LiChrosorb are used by chromatographers all over the world for many applications.

What is C8 and C18 column?

C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.

What is L1 packing?

According to the United States Pharmacopeia (USP), L1 column packing is defined as “octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter.” Some USP methods specifying an L1 column use intermediate pH, and many of these methods were done on a 10 m, 3.9 x 300 mm column.

What is BDS in chromatography?

The term BDS stands for Base Deactivated Silica. Therefore, these columns are also named as endcap columns. This column is very important in chromatography because it has its residual silanol groups deactivated by capping.

What is BDS and ODS?

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

What is L3 packing in HPLC column?

L3 USP columns are described as consisting of porous silica particles, 1.5 to 10 µm in diameter. Our L3 USP columns are produced to the highest quality standards to ensure reliable, reproducible results. A perfect example is: Chromolith® Si.

What is ODS and BDS?

What is needle wash in HPLC?

Your Agilent autosampler is designed to deliver accurate measurements, precise injection volumes, low carryover, and high-quality data. Needle wash is often required to ensure minimum carryover in order to avoid ghost peaks, inaccurate quantitation, or other chromatographic issues.

What is priming in HPLC?

Before using an HPLC system, you should prime all of the lines in your HPLC pump. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it).