What are HLA transgenic mice?
What are HLA transgenic mice?
Transgenic mice that express human HLA molecules represent a unique in vivo experimental model for evaluating human immune system function. These models have been used to study the role of the human class I or class II restricted T cell repertoire in autoimmune disease, infectious disease, and vaccine development.
What is a double transgenic mouse?
The double transgenic mice (dTg) were obtained by mating: (i) transgenic mice expressing the hemagglutinin of influenza virus under the insulin promoter with (ii) transgenic mice expressing specific T lymphocytes with receptor for the immunodominant epitope of the same virus.
What is the difference between transgenic mice and knockout mice?
Two important tools used by researchers include transgenic mice, in which a foreign gene is integrated into an animal’s genetic material, and knockout/knock-in mice, in which targeted genes either are rendered nonfunctional or are altered.
What are smarta mice?
These mice have a CD4+ T cell repertoire that is specific for the LCMV GP61-80 epitope. SMARTA-1 mice exhibit differently skewed T cell populations, with a 27:1 ratio of single positive CD4:CD8 thymocytes. Hemizygous mice are viable and fertile.
What are App PS1 transgenic mice?
APP/PS1 are double transgenic mice expressing a chimeric mouse/human amyloid precursor protein (Mo/HuAPP695swe) and a mutant human presenilin 1 (PS1-dE9), both directed to CNS neurons. Both mutations are associated with early-onset Alzheimer’s disease.
What are transgenic mice used for?
They have been used to study and model obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging, temperature and pain reception, and Parkinson disease. Transgenic mice generated to carry cloned oncogenes and knockout mice lacking tumor suppressing genes have provided good models for human cancer.
Why do we use transgenic mice?
Transgenic mice can also be used to study gene function or to generate models for human genetic disease, provided that the desired effect can be observed when the transgene is expressed in the presence of the multitude of host genes. In other words, dominant gain-of-function genetic alterations can be studied.
What are P14 cells?
Model Description. The Tcrα Knockout/Transgenic LCMV P14 T Cell Receptor line is homozygous for a transgene that encodes a T cell receptor that is specific for a peptide (P14) from the lymphocytic choriomeningitis virus (LCMV) presented by the MHC class I molecule H-2Db.
What are HLA class 1 and 2?
HLA class I molecules are expressed on the surface of almost all nucleated cells. Class II molecules are expressed only on B lymphocytes, antigen-presenting cells (monocytes, macrophages, and dendritic cells), and activated T lymphocytes.
Does everyone have HLA AB and C?
It has been shown that high resolution HLA typing (HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1 and HLA-DPB1) may be relevant in transplantation to identify a full match, even when the donor is related….In graft rejection.
| HLA allele | Diseases with increased risk | Relative risk |
|---|---|---|
| HLA-DQ2 and HLA-DQ8 | Coeliac disease | 7 |
How much is a transgenic mouse?
Transgenic Core Cost Estimates & Fees
| Service Offered | Cost |
|---|---|
| B6SJLF2 Hybrid Transgenic Mouse Production | $3,700.00 |
| C57BL/6J Transgenic Mouse Production | $5,800.00 |
| Custom Genetic Background Transgenic Mouse Production | $6,800.00 |
| Sprague Dawley Outbred Transgenic Rat Production (other strains available) | $11,000.00 |
What are APP mice?
APPPS1 mice contain human transgenes for both APP bearing the Swedish mutation and PSEN1 containing an L166P mutation, both under the control of the Thy1 promoter. In these mice, expression of the human APP transgene is approximately 3-fold higher than endogenous murine APP.
How long do APP ps1 mice live?
In mice with seizures a 10-15% mortality is reported on the congenic (N9) C57BL/6 background. In these mice, sudden deaths occur at any age but peak around 3-4 months (Minkeviciene et al. J Neurosci. 2009).
How are transgenic mice made?
Typically, transgenic mice are generated by microinjecting the transgenic construct into a fertilized egg (oocyte or zygote). An alternative way to effectively introduce a transgene into an egg is the use of a retrovirus vector.
What is P14 mice?
Origin: The Transgenic LCMV-P14 T Cell Receptor transgenic mouse was made by Dr. Hanspeter Pircher and colleagues in 1989 at the Department of Experimental Pathology in Zurich, Switzerland. The transgenic line was created by coinjection of the P14 Tcrα and Tcrβ gene constructs into (C57BL/6 x DBA/2)F2 fertilized eggs.