Can you transfect primary cells?
Can you transfect primary cells?
Transfection of primary cells and stem cells is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
What is the best transfection reagent?
Under the tested conditions, ViaFect™ Reagent offered the best combination of transfection efficiency and low toxicity for most cell lines, making it an ideal choice when beginning transfection experiments with a new cell line.
What are transfection reagents?
Transfection reagents are used to introduce naked or purified nucleic acids into eukaryotic cells. These products may include plasmids, reagents, kits, primer sets, and more.
Why is it hard to transfect primary cells?
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with plasmid DNA. This is mainly due to their fragility and slow-dividing rates.
How do you transfect cells?
The physical transfection methods are the most recent methods and use diverse physical tools to deliver nucleic acids. The methods include direct micro injection, biolistic particle delivery, electroporation, and laser-based transfection [13].
What is transfection by chemical method?
Transfection methods include various approaches (physical and chemical methods) that are carried out by non-viral techniques – electroporation, calcium phosphate exposure, liposome-based transfection that allow to deliver cargo molecules through cellular membrane without any permanent damage to the cell.
How do you choose a transfection reagent?
Abstract. Optimal conditions must be determined experimentally when transfecting cultured cells. An ideal reagent should offer high transfection efficiency and low toxicity across a broad range of cell types.
What is the difference between lipofectamine 2000 and 3000?
Lipofectamine 3000 reagent yields higher transfection efficiencies than Lipofectamine 2000 reagent when tested in a variety of cell lines.
Are HeLa cells easy to transfect?
Even though HeLa cells are very hardy, the HeLa cell line is fairly easy to transfect and very receptive to viral vectors, making it a popular research tool in virology.
What are transfection methods?
Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.
Why is opti-MEM used for transfection?
OPTI-MEM is the best option since transfection efficiency is high when cells are grown in O-MEM during transfection. FBS in regular media hinders transfection so using Opti-MEM is good option when using Lipofectamine.
What is Lipofectamine reagent?
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection.
Which PEI is used for transfection?
polycation polyethylenimine
The polycation polyethylenimine (PEI) is one of the most utilized reagents for small- to large-scale transfections as it is simple to use and, when combined with optimized expression vectors and cell lines, provides high transfection efficiency and titers.
Why use transfex transfection reagent?
TransfeX Transfection Reagent has been optimized for use on a wide range of cell types, including cells that are generally difficult to transfect, such as hTERT immortalized cell lines, primary cells, and stem cells. TransfeX has been optimized for high transfection efficiency and low cytotoxicity, with reliability and scalability.
What is cationic lipid transfection?
Cationic lipid transfection is a fast, simple, and reproducible process for introducing DNA, RNA, or other oligonucleotides into eukaryotic cells. This classic method offers highly efficient transfection for a broad range of cell types, including adherent and suspension cell lines, as well as many primary cells and stem cells.
What is ATCC transfection reagent?
ATCC offers a line of transfection reagents tailored for effective nucleic acid transfer into a wide variety of cells for a multitude of applications. We have optimized several protocols using GeneX Plus and/or TransfeX for transfection. Click on the ATCC cell line to obtain the protocols.
Are there any commercial reagents for transfection of primary cells?
Fortunately, a number of commercial reagents have been demonstrated to successfully transfect a range of primary cells such as neurons, HUVECs, melanoma cells, among other difficult-to-transfect cells. Your search returned 70 Transfection Primary Cell Transfection Reagents across 12 suppliers.