What is allele-specific PCR used for?
What is allele-specific PCR used for?
Allele-specific polymerase chain reaction (ASPCR) is an application of the polymerase chain reaction (PCR) that permits the direct detection of any point mutation in human DNA by analyzing the PCR products in an ethidium bromide-stained agarose or polyacrylamide gel.
What is allele-specific amplification?
Allele-specific polymerase chain reaction (AS-PCR), also known as amplification refractory mutation system (ARMS) or PCR amplification of specific alleles (PASA) is a PCR-based method which can be employed to detect the known SNPs [7]. The concept of AS-PCR was initiated by Newton et al.
What is ARMS PCR or allele-specific PCR?
The amplification-refractory mutation system (ARMS) is a simple method for detecting any mutation involving single base changes or small deletions. ARMS is based on the use of sequence-specific PCR primers that allow amplification of test DNA only when the target allele is contained within the sample.
What is gene specific PCR?
Gene-specific primers are ready-to-use components of PCR and qPCR that enable the detection of specific genes. Eliminating the need for manual primer-design, these commercially prepared oligonucleotides can speed up molecular experiments. These usually come in primer pairs, containing forward and reverse sequences.
What is allele specific expression?
Allele-specific expression (ASE) refers to the characteristic of preferentially expressing a parental allele in the hybrid due to variations in regulatory sequences from the parental genomes (11). The expression difference caused by ASE may lead to phenotypic variation depending on the function of the genes.
What are allele specific primers?
Allele Specific Primer Extension (ASPE) is a solution based, sequence specific enzymatic reaction technology that can be used to assay multiple SNPs in a single tube.
How do you make allele specific primers?
Allele Specific Primer Design Guidelines
- ASPE primers should be from the same DNA strand (per target sequence) and be synthesized for all the sequence variants.
- The Ta opt should be around 51-56 °C.
- The SNP should be present at the 3′ end of the allele specific primer for accurate hybridization.
What are two different alleles called?
Each pair of alleles represents the genotype of a specific gene. Genotypes are described as homozygous if there are two identical alleles at a particular locus and as heterozygous if the two alleles differ. Alleles contribute to the organism’s phenotype, which is the outward appearance of the organism.
What is SNP in biology?
Listen to pronunciation. (snip) A DNA sequence variation that occurs when a single nucleotide (adenine, thymine, cytosine, or guanine) in the genome sequence is altered and the particular alteration is present in at least 1% of the population. Also called single nucleotide polymorphism.
What is the difference between a SNP and a mutation?
SNP is a change in the single-nucleotide of a genome. Also, it is a type of mutation. Mutation is the variation in DNA base pairs caused due to insertion, deletion, duplication or substitution of base pairs. The variation is seen only in a single nucleotide.
What allele means?
An allele is a variant form of a gene. Some genes have a variety of different forms, which are located at the same position, or genetic locus, on a chromosome. Humans are called diploid organisms because they have two alleles at each genetic locus, with one allele inherited from each parent.
How many alleles are in a gene?
two alleles
When the copies of a gene differ from each other, they are known as alleles. A given gene may have multiple different alleles, though only two alleles are present at the gene’s locus in any individual.